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Hidden Fungal DNA Structures May Shape Sequencing Outcomes
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DOI：10.1002/bies.70153.png)
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Abstract
En 中文
Fungal DNA is systematically under-detected in shotgun metagenomics, likely due in part to physical barriers like melanized cell walls and complex DNA conformations. Additionally, Oxford Nanopore Technologies sequencing with native fungal DNA often results in rapid pore clogging and unusual translocation dynamics, possibly due to intrinsic, yet undescribed, structural complexities. Exploring these signals could reveal novel fungal genome architectures, enhance sequencing accuracy, and drive advances in fungal biology.
Keywords:
DNA
fungi
nanopore
novel structures
sequence artefacts
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