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High-throughput multiplexed fluorescence-activated droplet sorting
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DOI：10.1038/s41378-018-0033-2.png)
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Abstract
En 中文
Fluorescence-activated droplet sorting (FADS) is one of the most important features provided by droplet-based microfluidics. However, to date, it does not allow to compete with the high-throughput multiplexed sorting capabilities offered by flow cytometery. Here, we demonstrate the use of a dielectrophoretic-based FADS, allowing to sort up to five different droplet populations simultaneously. Our system provides means to select droplets of different phenotypes in a single experimental run to separate initially heterogeneous populations. Our experimental results are rationalized with the help of a numerical model of the actuation of droplets in electric fields providing guidelines for the prediction of sorting designs for upscaled or downscaled microsystems.
Keywords:
PROTEIN-SIGNALING NETWORKS
MAMMALIAN-CELLS
MICROFLUIDICS
MANIPULATION
SELECTION
BREAKUP
ASSAYS
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