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Highly sensitive luciferase-based assay with red fluorescent protein expression for accurate quantitative monitoring and real-time visualization of cell invasion
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DOI：10.1016/j.ab.2025.115986.png)
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Abstract
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• Cells co-expressing luciferase and red fluorescent protein were generated. • Luciferase measurements and cell count correlate, validating its application. • Luciferase activities were used to accurately quantify variations in cell invasion. • Red fluorescent protein dTomato provided real-time visualization of invasion. • Our established assay showed high sensitivity for measuring invading cells.
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