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hsa_circ_0005963 downregulation suppresses NSCLC malignant progression by miR-506-3p/SOX9-mediated stemness regulation
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DOI：10.1080/17501911.2026.2654577.png)
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Abstract
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This study investigated the role and regulatory mechanisms underlying hsa_circ_0005963 in NSCLC.
FISH and RT-qPCR were used to assess hsa_circ_0005963 expression in NSCLC cells and tissues. Dual-luciferase reporter assay was employed to characterize hsa_circ_0005963 downstream targets. Transwell migration and EdU incorporation were performed to detect NSCLC cell proliferation and migration. A mouse tumor xenograft model was constructed to assess hsa_circ_0005963 functions in NSCLC metastasis and progressions.
hsa_circ_0005963 expression was upregulated in NSCLC tissues and cells. High hsa_circ_0005963 expression indicates poor prognosis, including overall survival. hsa_circ_0005963 silencing inhibited NSCLC proliferation and migration. Experiments confirmed that hsa_circ_0005963 downregulation suppresses A549 tumor invasions. A dual-luciferase reporter assay identified SOX9 and microRNA as hsa_circ_0005963 downstream targets, which was validated by RT-qPCR. SOX9 overexpression or miR-506-3p downregulation reversed the hsa_circ_0005963 silencing inhibitory effects upon proliferation and migrations. SOX9 overexpression reversed miR-506-3p effects to inhibit proliferation and migration in both cell lines.
hsa_circ_0005963 promotes NSCLC development by sponging miR-506-3p to enhance SOX9 expression.
Keywords:
hsa_circ_0005963
NSCLC
miR-506-3p
SOX9
cancer stem cells
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