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Identification of novel major quantitative trait loci associated with selenium and zinc accumulation in Aegilops tauschii

delete2026-08-12
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OA
AI
H
HC Hong Chen
G
GW Guoqing Wang
W
WT Wenhui Tian
S
SN Shunzong Ning
D
DL Dengcai Liu
L
LZ Lianquan Zhang
B
BZ Bo Zhang *
T
TL Tao Liu *
DOI:10.3389/fpls.2026.1892742delete
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Abstract

Abstract

En 中文
Crop selenium and zinc biofortification relies heavily on bioaccumulation efficiency; whereas the genetic and molecular mechanisms mediating selenium (Se) and zinc (Zn) accumulation in Aegilops tauschii are still poorly understood. In this study; quantitative trait locus (QTL) mapping was carried out for selenium and zinc content in a recombinant inbred line (RIL) population derived from a cross between AS2407 (Aegilops tauschii subsp. strangulata) and AS65 (Aegilops tauschii subsp. tauschii). Two major QTLs; QSe-1D and QZn-2D; controlling Se and Zn content were identified on chromosomes 1D and 2D; respectively. QSe-1D was mapped to a 0.5 cM interval with 11.94% phenotypic variance explained (PVE); while QZn-2D was located within a 1.1 cM interval and explained 13.49% PVE. Through an integrated analysis of functional annotation; expression data; and conserved domain variations; we identified three putative genes linked to indirect selenium accumulation mechanisms and three candidates involved in zinc metabolism as the most promising targets. SSR markers (SSR-1D7 and SSR-2D2) closely linked to these two QTLs were developed; and their predictive efficiency was validated in a natural population consisting of 126 Ae. tauschii accessions. These findings contribute to deciphering the genetic basis of Se and Zn accumulation; offering theoretical references for marker-assisted selection in biofortification breeding.
Keywords:
metabolism
zinc
selenium
molecular markers
QTL
Aegilops tauschii

Journal

Frontiers in Plant Science cover
Frontiers in Plant Science
IF:
4.8
Papers:
3.4W
Citations:
14.7W

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