.png){kind=icon}
.png){kind=icon}
Return
Image acquisition for colocalization using optical microscopy
.png){kind=icon}
PRE
AI
DOI：10.1152/ajpcell.00133.2008.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Colocalization, in which images of two or more fluorescent markers are overlaid, and coincidence between the probes is measured or displayed, is a common analytical tool in cell biology. Interpreting the images and the meaning of this identified coincidence is difficult in the absence of basic information about the acquisition parameters. In this commentary, we highlight important factors in the acquisition of images used to demonstrate colocalization, and we discuss the minimum information that authors should include in a manuscript so that a reader can interpret both the fluorescent images and any observed colocalization.
Keywords:
RYANODINE RECEPTORS
CONFOCAL IMAGES
DECONVOLUTION
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
A
IF:
4.7
Papers:
7.2K
Citations:
1.7W