.png){kind=icon}
.png){kind=icon}
Return
Immunolocalization of the β-O-4 linked structure of lignin in wood and bamboo cell walls using a novel monoclonal antibody
.png){kind=icon}
PRE
AI
Y
M
K
S
Y
N
T
R
Y
K
T
F
K
K
Y
T
DOI：10.1515/hf-2025-0141.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
A mouse monoclonal antibody was generated against a beta-O-4 oligomer-p-aminohippuric acid-(pAHA)-bovine serum albumin (BSA) conjugate. Competitive ELISA showed that the antibody reacted specifically with beta-O-4 oligomer conjugate and did not react with other dimeric model compound conjugates containing the beta-5, beta-beta, or 5-5 linkages. This antibody reacted with both the beta-O-4 linked guaiacyl and syringyl structures. In Chamaecyparis obtusa, labeling was strong in the secondary walls (SW), and weak in the compound middle lamella (CML). In Betula grossa, labeling was strong in the outer SW of wood fibers and uniform in the SW of vessel elements and the inner part of the SW of wood fibers. In contrast, labeling was strong in wood fibers rich in syringyl lignin in Quercus crispula and weak in CML, including cell corners, the SW of vessel elements, and vasicentric tracheids rich in guaiacyl lignin. In Phyllostachys edulis, labeling was uniform in developing fiber SW and stronger in inner part in developed fiber SW. The localization patterns were like those of feruloyl arabinoxylan epitopes. This novel antibody will be an effective tool to immunolocalize the beta-O-4 linked structure of lignin in plant cell walls.
Keywords:
Chamaecyparis obtusa
Betula grossa
lignin
Quercus crispula
Phyllostachys edulis
beta-O-4 linkage
Journal
H
IF:
1.6
Papers:
37
Citations:
4.6K
Organization
Cited Papers