.png){kind=icon}
Return
Improving single-cell transcriptome sequencing efficiency with a microfluidic phase-switch device
.png){kind=icon}
OA
AI
DOI：10.1039/c9an00823c.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
In this paper, we present a novel method to improve the efficiency of single-cell transcriptome sequencing for analyzing valuable cell samples. The microfluidic device we designed integrates multiple singlecell isolation chambers with hydrodynamic traps and achieves a nearly 100% single-cell capture rate and minimal cell loss, making it particularly suitable for samples with limited numbers of cells. Single cells were encapsulated using a novel phase-switch method into picoliter-sized hydrogel droplets and easily recovered for subsequent reactions. Minimizing the reaction volume resulted in a high reverse transcription (RT) efficiency for RNA sequencing (RNA-Seq). With this novel microfluidic platform, we captured dozens of hESCs (H9) simultaneously and obtained live cells in individual picoliter volumes, thus allowing for the convenient construction of a high-quality library for deep single-cell RNA-Seq. Our single-cell RNA-Seq results confirmed that a spectrum of pluripotency existed within an H9 colony. This integrated microfluidic platform can be applied to various cell types for the investigation of rare cellular events, and the phase-switch single-cell processing strategy will improve the efficiency and accessibility of single-cell transcriptome sequencing analysis.
Keywords:
CIRCULATING TUMOR-CELLS
EMBRYONIC STEM-CELLS
GENE-EXPRESSION
HETEROGENEITY
LINES
PLURIPOTENCY
MAINTAIN
GROWTH
DNA
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
A
IF:
3.3
Papers:
1.6W
Citations:
3.3W
Organization
View more organizations
Cited Papers
ShareSaveMutation Analysis of Cell-Free DNA and Single Circulating Tumor Cells in Metastatic Breast Cancer Patients with High Circulating Tumor Cell Counts.png)
CLINICAL CANCER RESEARCHOAAI
CLINICAL CANCER RESEARCHIF10.2
OAAIShareSaveShareSaveShareSaveAn automated microfluidic device for assessment of mammalian cell genetic stabilityLAB ON A CHIPOAAI
LAB ON A CHIPIF5.4
OAAIShareSaveHigh-throughput spatial mapping of single-cell RNA-seq data to tissue of originNATURE BIOTECHNOLOGYOAAI
NATURE BIOTECHNOLOGYIF41.7
OAAIShareSaveShareSaveShareSave View more