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In Silico Study of Molecular Docking Between Lactoferrin and Autophagy-apoptosis Genes ATG3, ATG5, and ATG12 and In Vitro Study of Lactoferrin Effect on These Gene Expressions in Breast Cancer
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DOI：10.1002/jcb.70072.png)
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Abstract
En 中文
Cancer is a major global health problem. Considering the special role of lactoferrin (Lf) in inhibiting the growth of cancer cells, a new approach has emerged for cancer treatment. The present study aimed to investigate the effect of Lf on the expression level of autophagy-apoptotic genes ATG5, ATG3, and ATG12 in breast cancer cell line MCF7 and an in silico study of the interaction between lactoferrin and the promoter of these genes and their proteins. The survival percentage of MCF7 cells in concentrations of 200, 300, and 500 mu g was 94.4%, 91.64%, and 52%, respectively. The expression level of the ATG3 gene in treatment with different concentrations of Lf was increased, but for the ATG5 gene, it decreased. The expression level of the ATG12 gene in cancer cells treated with Lf concentrations of 200 and 300 mu g/ml was increased; however, it decreased at the concentration of 500 mu g/ml. All concentrations significantly differed from the control (p < 0.05). The interaction of lactoferrin with ATG3, ATG5, and ATG12 proteins showed that the N-lobe region of the lactoferrin interacted with the N-lobe, catalytic domain, and the HR domain of the ATG3 protein, with the HR domain and the UblB domain of the ATG5 protein, and with the C domain of the ATG12 protein. Analysis of HADDOCK data showed that Lf interacted with the promoter of the genes. Lf, in addition to its effect on the expression of the genes, also interacts with the promoter of genes and their protein.
Keywords:
apoptosis
ATG12
ATG3
ATG5
autophagy
bioinformatics
breast cancer
lactoferrin
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