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In vitro cytocompatibility, antibacterial properties, and hemocompatibility of MAO-Zn/PLA composite films for potential guided bone regeneration treatments
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DOI：10.1088/1748-605X/ae6214.png)
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Abstract
En 中文
To evaluate the application potential of micro-arc oxidized Zn foil (MAO-Zn)/Poly(lactic acid) (PLA) composite films in guiding oral bone regeneration (GBR) from the perspective of material composition, their cytocompatibility, antibacterial properties, and hemocompatibility were investigated. The cytocompatibility was evaluated using human gingival fibroblasts (HGFs) and MC3T3-E1 cells by the CCK-8 method. For PLA films, the viabilities of the above two types of cells were 87.6% and 84.5%, respectively, and their morphology was normal. However, PLA has no antibacterial ability. In contrast, the inhibition rates of Zn foil and MAO-Zn foil against S. aureus reached 48.56% and 48.19%, respectively, and their inhibition rates against Porphyromonas gingivalis reached 76.6% and 80.99%, respectively, after co-culturing with the bacterial suspension for 24 h. The cell viabilities of these two groups were only 10.38% and 9.13% for HGF and 67.31% and 8.90% for MC3T3-E1 cells, respectively, after they were cultured with the original extract for 5 d. The abnormal morphology also implied their toxicity. Under the protection of PLA for the composite film, direct contact between Zn foil or MAO-Zn foil and surrounding cells was avoided, and long-term sustained release of Zn2+ was also achieved. The bacterial inhibition rates of the composite film were 81.09% and 89.85%, respectively. Meanwhile, the morphology of the above two types of cells was normal after they were cocultured on the surface of the composite film for 24 h. Moreover, the expression levels of osteogenesis-related genes (COLL-alpha, OCN, ALP, and RUNX2) increased. The hemolysis rate of the composite film was less than 2%. The cell viability in the 1:9 diluted extract was 91.54% and 87.46%, while that in the original extract was only 8.75% and 3.36%, respectively. It is still necessary to further reduce its toxicity by subsequently adjusting its composition and structure. This study provides an experimental basis and theoretical reference for further enhancing the application potential of this kind of composite film in the field of GBR membranes.
Keywords:
Zn foil
micro-arc oxidation
poly(lactic acid)
composite films
cytocompatibility
antimicrobial properties
hemocompatibility
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