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Information-rich interferometric point-spread function for type-B 4Pi localization microscopy
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DOI：10.1364/OE.534190.png)
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Abstract
En 中文
The 3D location of light emitters provides essential information in many biological, chemical and physical systems. 4Pi single-molecule localization microscopy (SMLM) being one of the state-of-the-art techniques provides exceptional precision in the axial direction; however, the lateral precision degrades significantly when the emitter is displaced several wavelengths away from the mutual focal plane. Here we propose a hybrid system employing type-B 4Pi microscope and engineered point-spread function (PSF), the resultant interferometric PSF is optimized against the Cramer-Rao metric, yielding the richest information regarding the emitter's 3D locations. It outperforms conventional 4Pi SMLM with a larger axial range and compares advantageously to the non-interferometric PSFs with doubled photon efficiency and better precision in all directions.
Keywords:
LARGE AXIAL RANGES
TRACKING
Journal
IF:
3.3
Papers:
6.1W
Citations:
14.3W