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Intervertebral Disc-on-a-ChipMF: A New Model for Mouse Disc Culture via Integrating Mechanical Loading and Dynamic Media Flow
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DOI：10.1002/admt.202300606.png)
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Abstract
En 中文
This study aims to develop an ex vivo organ-on-a-chip model, intervertebral Disc-on-a-Chip(MF), to investigate integrated effects of mechanical loading and nutrition on disc health. The system consists of a detachable multilayer microfluidic chip, a Computer-Arduino-based control system, and a mechanical loading unit, which are optimized for accurate axial force measurement and the maintenance of a 21-day ex vivo disc culture. To ensure accuracy of axial force, the axial mechanical loading regimen is optimized, using the Computer-Arduino-based system and low-profile force sensors (LPFS) to control the mechanical loading unit, and the force distribution on the disc surface is modeled by computational simulation. A 21-day ex vivo disc culture is demonstrated using the Disc-on-a-Chip(MF) system, with optimized mechanical loading (0.02 MPa at 1Hz, 1.5 hr day(-1)) and flow rate (1 mu L min(-1)). The structural integrity, collagen breakdown, catabolic enzyme activities, and disc cell and collagen alignment reveal that the on-chip cultured discs exhibit a preferred disc health similar to that of native discs for up to 21 days, while discs in a static culture show degenerative changes. The mouse Disc-on-a-Chip(MF) system mimics in vivo disc microenvironment and provides a valuable platform for studying the effects of various factors on disc health and degeneration and testing new therapies.
Keywords:
bioreactors
intervertebral disc degeneration
mechanical loading
microfluidic
organ-on-a-chip
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