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Intrinsic waste component synergy: calcium-rich eggshell waste modulates fungal-bacterial microbiome toward selectively medium-chain fatty acid production
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DOI：10.1016/j.biortech.2026.134795.png)
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Abstract
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• The optimal eggshell dosage for chain elongation (CE) was 20 g/L (EG-20). • 22.3 g COD/L caproate and 11.3 g COD/L endogenous ethanol was produced in EG-20. • Eggshells created beneficial microbial niches and provided sufficient butyrate. • CE bacteria were enriched to 10.2% compared to their absence in the control. • Ca2+ alleviated yeast inhibition on CEB in the absence of sugar.
Keywords:
eggshell waste
calcium ions
microbial niche
chain elongation
medium-chain fatty acids
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