.png){kind=icon}
.png){kind=icon}
Return
Light-activated; highly efficient intracellular biomolecular delivery using a titanium nitride micro-array device
.png){kind=icon}
PRE
AI
N
S
A
M
T
DOI：10.1039/D6AN00474A.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
This study investigates the effectiveness of optoporation; activated by a titanium nitride micro-array device; in transporting a wide range of biomolecules into cells. Titanium nitride is a propitious plasmonic material exhibiting localised surface plasmon resonances within the near-infrared biological transparency window. Thin films of titanium nitride were deposited on a glass substrate via sputtering; and a periodic TiN micro-array device was fabricated (1 cm × 1 cm) through photolithography and chemical etching. When the device is irradiated with a laser at 1064 nm with a laser fluence of 10.28 mJ cm−2 and a motorised scanning speed of 5 mm s−1 in the presence of biomolecules; photothermal bubbles form near the plasma membrane and temporarily create pores that facilitate the smooth entry of biomolecules into the cells. Our TiN micro-array device with a laser-scanning setup can transfect more than a million cells within 1 minute. Utilising this device; a variety of biomolecules; including propidium iodide (PI) dye (668.4 Da); EGFP-plasmid DNA (229.4 kDa); and β-galactosidase enzyme (465 kDa); were efficiently delivered into several mammalian cell lines (L929; SiHa; and NIH/3T3); achieving high delivery efficiency and excellent cell viability. The results highlight that the maximum delivery efficiency for the PI dye is 95%; and the cell viability reaches 98% in L929 cells. Similarly; for large molecules like the β-galactosidase enzyme; the delivery efficiency is as high as 94%; with 97% cell viability. The MTT assay confirmed that the device exhibits no cytotoxicity during cell transfection. Thus; it holds potential for applications in cell therapy and diagnostics.
Journal
A
IF:
3.3
Papers:
1.6W
Citations:
3.3W
Organization
Cited Papers