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Long-term stable tetrahedral DNA functionalized upconversion nanoparticles for point-of-care detection of neutrophil gelatinase-associated lipocalin
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DOI：10.1016/j.cclet.2025.112159.png)
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Abstract
En 中文
Achieving point-of-care detection of neutrophil gelatinase-associated lipocalin (NGAL) contributes to the early diagnosis and treatment of acute kidney injury (AKI), but the lack of highly sensitive and longterm stable detection methods is a major challenge. In this work, tetrahedral DNA (tDNA)-functionalized large-sized upconversion nanoparticles (t-UCNP300) were synthesized with high luminescence intensity. Leveraging the dual effects of surface charge density and the rigid structure of tDNA, the developed t-UCNP30 0 exhibited long-term stability for over 60 days after being stored in phosphate buffer at 25 degrees C and pH 7.4, with no obvious aggregation and only slight luminescence attenuation. By using this developed t-UCNP300, a luminescent lateral flow immunoassay (LFIA) strip was developed for point-of-care detection of NGAL, with a detection linearity range of 2.00-250 ng/mL and limit of detection (LOD) of 1.00 +/- 0.07 ng/mL (S/N = 3). Taking advantage of the developed LFIA strip for NGAL with high selectivity and a rapid response time of < 5 min, it was successfully applied for the determination of NGAL in artificial urine samples. (c) 2026 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
Keywords:
Upconversion nanoparticles
Tetrahedral DNA
Lateral flow immunoassay
NGAL detection
Point-of-care detection
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8.9
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1.2W
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3.8W
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