.png){kind=icon}
Return
Macrolide Biosensor Optimization through Cellular Substrate Sequestration
.png){kind=icon}
OA
AI
DOI：10.1021/acssynbio.0c00572.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Developing and optimizing small-molecule biosensors is a central goal of synthetic biology. Here we incorporate additional cellular components to improve biosensor sensitivity by preventing target molecules from diffusing out of cells. We demonstrate that trapping erythromycin within Escherichia coli through phosphorylation increases the sensitivity of its biosensor (MphR) by approximately 10-fold. When combined with prior engineering efforts, our optimized biosensor can detect erythromycin concentrations as low as 13 nM. We show that this strategy works with a range of macrolide substrates, enabling the potential usage of our optimized system for drug development and metabolic engineering. This strategy can be extended in future studies to improve the sensitivity of other biosensors. Our findings further suggest that many naturally evolved genes involved in resistance to multiple classes of antibiotics may increase intracellular drug concentrations to modulate their own expression, acting as a form of regulatory feedback.
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
3.9
Papers:
3.9K
Citations:
1.2W
Organization
Cited Papers
Genetic engineering of macrolide biosynthesis: past advances, current state, and future prospects
PREAI
ShareSaveShareSaveGeographic distribution of Angiostrongylus cantonensis in wild rats (Rattus rattus) and terrestrial snails in Florida, USA.png)
PLOS ONE
OAAI
PLOS ONEIF0
OAAIShareSaveHistaminergic Control of Oxytocin Release in the Paraventricular Nucleus during Lactation in Rats
PREAI
ShareSaveShareSave
View more