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Method for trapping and manipulating nanoscale objects in solution
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DOI：10.1063/1.1872220.png)
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We present a device that allows a user to trap a single nanoscale object in solution at ambient temperature, and then to position the trapped object with nanoscale resolution. This anti-Brownian electrophoretic trap (ABEL trap) works by monitoring the Brownian motion of the particle (via fluorescence microscopy), and then applying a feedback voltage to the solution so that the electrophoretic drift exactly cancels the Brownian motion. The ABEL trap works on any object that can be imaged optically and that acquires a charge in water. The ABEL trap is noninvasive, is gentle enough to handle biological molecules, and can trap objects far smaller than can be trapped with laser tweezers. Our proof-of-principle device can trap fluorescent polystyrene nanospheres with diameters down to 20 nm. (C) 2005 American Institute of Physics.
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BIOPHYSICAL JOURNAL
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BIOPHYSICAL JOURNALIF3.1
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PROCEEDINGS OF THE IEEEIF25.9
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