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Microbial single-cell sequencing technologies: Principles, applications, and spatial integration
DOI:10.1016/j.biotechadv.2026.108996.png)
Abstract
En 中文
• Single-cell sequencing resolves microbial heterogeneity beyond bulk omics methods. • Split-pool barcoding, FACS, and droplet microfluidics serve diverse isolation needs. • Microbial scRNA-seq uncovers transcriptional diversity and dynamic cellular states. • Microbial scDNA-seq enables strain-level genome recovery and rare variant detection. • Spatial omics integrates sequencing and FISH to map microbial communities in situ.
Keywords:
Microbial single-cell sequencing
Microbial ecosystems
Spatial omics
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