.png){kind=icon}
.png){kind=icon}
Return
Microinjection of Synthetic Peptides Into Caenorhabditis elegans
.png){kind=icon}
PRE
AI
O
T
Y
DOI：10.21769/BioProtoc.5640.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
The genome of the nematode Caenorhabditis elegans encodes at least 160 predicted peptide precursor genes that can generate over 300 bioactive peptides, the functions of most of which remain unknown. Phenotypes resulting from deletion or transgenic expression of peptide genes are readily assayed, but genetic dissection of individual peptide activities is often confounded when a single gene encodes multiple peptides or when distinct peptides act redundantly. Here, we describe a protocol for direct microinjection of chemically synthesized peptides into individual worms. This approach permits investigation of the effects of an individual peptide while providing precise temporal control over peptide delivery.
Keywords:
Bioactive peptides
C. elegans
In vivo experiment
Microinjection
Chemical synthesis
Journal
B
IF:
1.1
Papers:
191
Citations:
5.4K
Organization
Cited Papers