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MicroRNA-encoding long non-coding RNAs
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DOI：10.1186/1471-2164-9-236.png)
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Abstract
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Background: Recent analysis of the mouse transcriptional data has revealed the existence of similar to 34,000 messenger-like non-coding RNAs (ml-ncRNAs). Whereas the functional properties of these ml-ncRNAs are beginning to be unravelled, no functional information is available for the large majority of these transcripts. Results: A few ml-ncRNA have been shown to have genomic loci that overlap with microRNA loci, leading us to suspect that a fraction of ml-ncRNA may encode microRNAs. We therefore developed an algorithm (PriMir) for specifically detecting potential microRNA-encoding transcripts in the entire set of 34,030 mouse full-length ml-ncRNAs. In combination with mouse-rat sequence conservation, this algorithm detected 97 ( 80 of them were novel) strong miRNA-encoding candidates, and for 52 of these we obtained experimental evidence for the existence of their corresponding mature microRNA by microarray and stem-loop RT-PCR. Sequence analysis of the microRNA-encoding RNAs revealed an internal motif, whose presence correlates strongly (R-2 = 0.9, P-value = 2.2 x 10(-16)) with the occurrence of stem-loops with characteristics of known pre-miRNAs, indicating the presence of a larger number microRNA-encoding RNAs (from 300 up to 800) in the ml-ncRNAs population. Conclusion: Our work highlights a unique group of ml-ncRNAs and offers clues to their functions.
Keywords:
POSTTRANSCRIPTIONAL REGULATION
LET-7 MICRORNA
NUCLEAR EXPORT
IDENTIFICATION
SEQUENCES
GENES
REAL
CLASSIFICATION
TRANSCRIPTION
EXPRESSION
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