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Microscope calibration using laser written fluorescence

delete2018-08-08
delete25
delete
OA
AI
A
Alexander D. Corbett *
M
Michael Shaw
A
Andrew Yacoot
A
Andrew Jefferson
L
Lothar Schermelleh
T
Tony Wilson
M
Martin J. Booth
P
Patrick S. Salter
DOI：10.1364/OE.26.021887delete
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Abstract

Abstract

En 中文
There is currently no widely adopted standard for the optical characterization of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and color plane alignment using confocal and structured illumination fluorescence microscopes. (C) 2018 Optical Society of America under the terms oldie OSA Open Access Publishing Agreement
Keywords:
STRUCTURED ILLUMINATION MICROSCOPY
TRANSPARENT MATERIALS
ABERRATION CORRECTION
OPTICAL MEMORY
RESOLUTION
IMAGES
GLASS
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Journal

Optics Express cover
Optics Express
IF:
3.3
Papers:
6.1W
Citations:
14.3W

Organization

N
national physical laboratory - uk
Scholars:
2.0K
Papers: 1.9K
Citations: 2
U
University of Exeter
Scholars:
2.0W
Papers: 2.1W
Citations: 3.6W
U
university of oxford
Scholars:
9.7W
Papers: 8.6W
Citations: 137
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