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miRNA-29a inhibits the proliferation of HUVECs by regulating the ITGB1/β-catenin/c-Myc pathway
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DOI：10.3892/mmr.2026.13825.png)
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Abstract
En 中文
Infantile hemangioma (IH) is a type of benign vascular tumor observed in younger patients. Previously, 216 differentially expressed microRNAs (miRs/miRNAs) associated with IH have been identified. In addition, common hub genes and miRNAs related to proteoglycan signaling pathways in angiogenesis and cancer have been identified, including c-Myc, integrin beta 1 (ITGB1), Bcl2 and miR-29a. Therefore, the present study aimed to explore the pathogenesis of IH from the perspective of previously identified miRNA gene network and protein-protein interactions. Gene and protein levels in human umbilical vein endothelial cells (HUVECs) were analyzed using reverse transcription-quantitative PCR and western blot (WB) analysis. Cell viability was assessed using a Cell Counting Kit-8 assay, and the potential association between miR-29a with ITGB1 was validated using a dual-luciferase reporter assay. The inhibition of ITGB1 suppressed the beta-catenin/c-Myc pathway in HUVECs. In addition, transfection with small interfering RNAs (siRNAs) targeting ITGB1 decreased the viability of HUVECs. Furthermore, siRNAs targeting mucin 1 and beta-N-acetylglucosaminidase significantly inhibited the c-Myc pathway in HUVECs. The results of WB and dual-luciferase reporter assays demonstrated that miR-29a regulated the beta-catenin/c-Myc pathway and the viability of HUVECs in HUVECs by directly binding to ITGB1. Therefore, miR-29a may serve as a potential therapeutic target for IH.
Keywords:
infantile hemangioma
c-Myc
human umbilical vein endothelial cells
integrin beta 1
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