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Modeling bacteriophage attachment using adsorption efficiency
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DOI：10.1016/j.bej.2012.02.007.png)
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Abstract
En 中文
Typically, models of bacteriophage adsorption consider the process in two steps: reversible and irreversible attachment. In this study, a recently introduced one-step adsorption model, the adsorption efficiency model, is used to describe the adsorption of T-series bacteriophages to Escherichia coli. The adsorption efficiency model simplifies phage attachment to a single step: irreversible binding.The adsorption efficiency (epsilon) is used to account for unadsorbed phages. The model accurately describes T-series phage adsorption (T2, T4, T5, T6, and T7) under a variety of conditions. In addition, the model is compared to a commonly used two-step adsorption model, the sequential model. Experimental data support the assumptions of the adsorption efficiency model and suggest that the reversible first step of T-series phage adsorption is equivalent to irreversible attachment under the conditions tested. The adsorption efficiency model was not appropriate for a phage lambda strain lacking side tail fibers. However, the model did agree with data previously published for a strain of phage lambda possessing side tail fibers, as is the case of all T-series strains tested. This suggests that the adsorption efficiency model applies to phages containing side tail fibers (C) 2012 Elsevier B.V. All rights reserved.
Keywords:
Bacteriophage
Adsorption
Adsorption efficiency
Modeling
Kinetic parameters
Dynamic simulation
Journal
IF:
3.8
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6.2K
Citations:
1.4W