Molecular characterization and preliminary functional analysis of the NLRP3 inflammasome in golden pompano (Trachinotus ovatus) in response to Streptococcus agalactiae infection

2026-08-05

PRE

Zhengjing Lin

Xuemei Mou

Peng Xu

Kianann Tan

Ting Huang

Renjie Li

Shuo Hu

Xiaoxin Huang

Sisi Huang

Lanfang Dong *

Xiaohui Cai *

DOI：10.1016/j.fsi.2026.111621

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Abstract

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• Five core NLRP3 inflammasome genes from Trachinotus ovatus were cloned and characterized. • Expression profiles of the genes were examined in liver, spleen, head kidney and intestine post S. agalactiae challenge. • ToNLRP3 was distributed in the cytoplasm, and ToASC was co-localized with ToCaspase-1 with obvious interaction. • Nigericin and ATP (NLRP3 inflammasome inducer/agonist) markedly upregulated ToNLRP3, ToASC and ToCaspase-1 expression.

Keywords:

Trachinotus ovatus

NLRP3 inflammasome

Streptococcus agalactiae

Innate immunity

ASC speck

Molecular characterization and preliminary functional analysis of the NLRP3 inflammasome in golden pompano (Trachinotus ovatus) in response to Streptococcus agalactiae infection

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