.png){kind=icon}
.png){kind=icon}
Return
Molecular determinants of sugar donor selectivity in MGD1, the major galactolipid synthase in Arabidopsis thaliana
.png){kind=icon}
PRE
AI
M
D
C
M
M
C
DOI：10.1016/j.biochi.2026.03.010.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Galactolipids are characteristic components of photosynthetic membranes, but the molecular bases explaining the marked preference of plastidial glycolipid synthases for galactose over glucose remains unknown. In Arabidopsis, MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE 1 (MGD1) catalyzes the bulk synthesis of monogalactosyldiacylglycerol (MGDG) and exhibits remarkable specificity for its sugar donor, UDP-galactose. MGD1 belongs to the large Glycosyltransferase family where it is classified in GT28 family. Here, using a structure-guided mutational approach, we dissected the molecular constraints governing the selectivity of sugar donor in this essential plastidial enzyme. We have identified two peptide regions and several residues essential for MGD1 catalysis, in particular a 'P-Q-E' motif acting as a core determinant of sugar donor recognition in galactolipid synthases. Variations in protein sequences around the proline residue allowed us to define distinct peptide signatures that discriminate galactolipid from glucolipid synthases in GT28 family. The introduction of a GGx (x being an aliphatic amino acid) or GAL sequence into MGD1 resulted in a mutant that exhibited glucosyltransferase activity. However, complete conversion to a glucose-specific enzyme could not be achieved without a significant loss of catalytic efficiency, revealing intrinsic constraints related to donor reprogramming. A comparative analysis with GT1 UDP-glycosyltransferases further highlighted the fundamental differences in how donor specificity is encoded in different glycosyltransferase families. Together, our results demonstrated that sugar donor specificity in glycolipid synthases emerges from distributed structural features rather than a single switch residue, and established bifunctional MGD1 variants as tools to probe the functional importance of galactolipids in chloroplast membrane biogenesis. (c) 2026 Elsevier B.V. and Soci & eacute;t & eacute; Fran & ccedil;aise de Biochimie et Biologie Mol & eacute;culaire (SFBBM). All rights are reserved, including those for text and data mining, AI training, and similar technologies.
Keywords:
Monogalactosyldiacylglycerol
Galactolipid synthases
Glucolipid synthases
Donor sugar specificity
Plant UGT
PSPG motif
Journal
IF:
3
Papers:
5.9K
Citations:
1.2W
Organization
Cited Papers