Monitoring Biomolecular Changes During Colitis Progression Using Infrared Spectroscopy in Dextran Sodium Sulfate-Treated Mouse Serum

2026-03-01

PRE

Mehmood Pirzada

Ekanayake, Sachini H.

Avash Kattel

Supriya Dhakal

Hemendra Ghimire

Nelson, Michael

Mathes Dayananda

Merlin, Didier

A G Unil Perera *

DOI：10.1002/jbio.70235

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Analysis of sequential alterations in body fluid constituents, such as serum, is vital for assessing inflammation. Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy was used to monitor biomolecular changes in a Dextran Sodium Sulfate (DSS) mouse colitis model. Serum samples collected on days 0, 3, and 7 post- (3% DSS) administration showed stepwise changes in lipid, protein, and carbohydrate regions. Statistically significant (SS) changes (p < 0.05) were detected at the initial stage of colitis (day 0 to 3) in six spectral biomarkers (SBMs), including the integral ratio of alpha-helix to beta-sheet (Amide I) and the beta-sheet (Amide I) to Tyrosine (Amide II). Changes intensified from day 3 to 7, with the most prominent differences from day 0 to 7, even when the initial changes (day 0 to 3) were not SS. These results highlight the potential of ATR-FTIR as a minimally invasive pre-screening tool for monitoring colitis progression.

Keywords:

INFLAMMATORY-BOWEL-DISEASE

IR SPECTROSCOPY

FTIR

MANAGEMENT

MARKERS

YOUNG

IBD

Monitoring Biomolecular Changes During Colitis Progression Using Infrared Spectroscopy in Dextran Sodium Sulfate-Treated Mouse Serum

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