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Multiplexed imaging to reveal tissue dendritic cell spatial localisation and function
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DOI：10.1002/1873-3468.14962.png)
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Abstract
En 中文
Dendritic cells (DCs) play a pivotal role in immune surveillance, acting as sentinels that coordinate immune responses within tissues. Although differences in the identity and functional states of DC subpopulations have been identified through multiparametric flow cytometry and single-cell RNA sequencing, these methods do not provide information about the spatial context in which the cells are located. This knowledge is crucial for understanding tissue organisation and cellular cross-talk. Recent developments in multiplex imaging techniques can now offer insights into this complex spatial and functional landscape. This review provides a concise overview of these imaging methodologies, emphasising their application in identifying DCs to delineate their tissue-specific functions and aiding newcomers in navigating this field. Dendritic cells (DCs) are crucial for the activation of adaptive immune responses and act in a tightly regulated in spatio-temporal manner. Recent technologies now allow investigating cells in their tissue environment. Here we discuss multiplexed imaging techniques and how they can help understand DC subtypes and their specific functions within tissue niches. image
Keywords:
dendritic cells
multiplexed imaging
spatial omics