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NIR-II multifocal structured illumination microscopy
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DOI：10.1364/OL.469259.png)
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Abstract
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Optical microscopy has been widely used as a versatile tool in biological research. However, its penetration depth and spatial resolution are desperately limited by light scat-tering during deep propagation in turbid medium. Here, we implement near-infrared second window (1000-1700 nm) multifocal structured illumination microscopy (NIR-II MSIM) capable of deep penetration, high contrast, and enhanced spatial resolution. Raster-scanning multifocal illu-mination patterns ensure homogeneous illumination of the sample. By integrating NIR-II photoemission into multifo-cal photoexcitation, NIR-II MSIM affords deep imaging with improved lateral resolution (similar to 1.49 mu m) at a depth of 2.5 mm in an Intralipid/agar phantom and outstanding contrast. Additionally, imaging at longer wavelength in the NIR-II region shows superior performance. This NIR-II MSIM system will afford a promising platform for studying phys-iological phenomena in turbid specimens in the future. (c) 2022 Optica Publishing Group
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