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Optimization of headspace sampling using solid-phase microextraction for volatile components in tobacco
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DOI：10.1016/S0021-9673(01)01376-0.png)
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Abstract
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Solid-phase microextraction (SPME) was evaluated as a tool for headspace sampling of tobacco samples. Several experimental parameters (e.g. sampling temperature, pH, moisture, and the type of SPME fibers) were optimized to improve sampling efficiency in two aspects; maximum adsorption and selective adsorption of volatile components onto SPME fibers. The effect of these parameters was often dominated by the physical and chemical nature (e.g. volatility, polarity) of target compounds, thus, SPME sampling conditions can be adjusted to favor a selected group of compounds, such as organic acids in tobacco. (C) 2002 Elsevier Science B.V. All rights reserved.
Keywords:
optimization
headspace sampling
tobacco
solid-phase microextraction
volatile compounds
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