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OsPATA1 deficiency enhances ABA-dependent salt tolerance by sequestering OsEULD1b in the cytosol of rice
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DOI：10.1016/j.cj.2025.07.007.png)
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Abstract
En 中文
Abiotic stresses, particularly salinity, pose a major threat to rice productivity, highlighting the need to identify novel genetic resources to improve stress tolerance. Gamma irradiation remains one of the most widely used tools breeding stress-tolerant plant varieties. In this study, we identified a salt-tolerant rice mutant, salt-insensitive TILLING line 4 (sitl4), generated via gamma irradiation and linked its enhanced tolerance to a loss-of-function mutation in Oryza sativa protein acyltransferase for ABA response 1 (OsPATA1), which encodes a DHHC-type palmitoyl acyltransferase. Functional analyses using both sitl4 and a CRISPR/Cas9-mediated OsPATA1-knockout line (ospata1) revealed that disruption of OsPATA1 leads to increased abscisic acid (ABA) accumulation and upregulation of ABA-responsive genes under salt stress conditions. We identified OsEULD1b, a previously uncharacterized Euonymus lectin (EUL) domain-containing protein, as an interactor of OsPATA1. In sitl4 and ospata1, OsEULD1b displayed cytosolic retention, suggesting that its subcellular redistribution enhances its role in ABA-mediated stress signaling. Taken together, our findings demonstrate that OsPATA1 and OsEULD1b form a regulatory module that modulates the ABA-dependent salt stress responses in rice. These results provide new insights into the molecular mechanisms underlying abiotic stress tolerance and will help to identify potential genetic targets for developing stress-tolerant rice cultivars through molecular breeding or genome editing.
Keywords:
DHHC-PAT
Mutation
Protein S-acylation
Rice
Salt stress
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