.png){kind=icon}
Return
Parameter estimation on multivalent ITC data sets
.png){kind=icon}
OA
AI
DOI：10.1038/s41598-022-17188-x.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
The Wiseman fitting can be used to extract binding parameters from ITC data sets, such as heat of binding, number of binding sites, and the overall dissociation rate. The classical Wiseman fitting assumes a direct binding process and neglects the possibility of intermediate binding steps. In principle, it only provides thermodynamic information and not the kinetics of the process. In this article we show that a concentration dependent dissociation constant could possibly stem from intermediate binding steps. The mathematical form of this dependency can be exploited with the aid of the Robust Perron Cluster Cluster Analysis method. Our proposed extension of the Wiseman fitting rationalizes the concentration dependency, and can probably also be used to determine the kinetic parameters of intermediate binding steps of a multivalent binding process. The novelty of this paper is to assume that the binding rate varies per titration step due to the change of the ligand concentration and to use this information in the Wiseman fitting. We do not claim to produce the most accurate values of the binding parameters, we rather present a novel method of how to approach multivalent bindings from a different angle.
Keywords:
TITRATION
CONSTANTS
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
3.9
Papers:
28.0W
Citations:
83.5W
Organization
Cited Papers
ShareSaveCharacterization of the kinetic and thermodynamic landscape of RNA folding using a novel application of isothermal titration calorimetry.png)
NUCLEIC ACIDS RESEARCHOAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAIShareSaveA coarse graining method for the identification of transition rates between molecular conformations
PREAI
ShareSaveShareSaveShareSaveMolecular cloning of the β‐subunit of the Na,K‐ATPase in the brine shrimp, ArtemiaFEBS LettersOAAI
FEBS LettersIF0
OAAIShareSaveMethod for determination of association and dissociation rate constants of reversible bimolecular reactions by isothermal titration calorimetersANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
ShareSave
no more
no more