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Parameter Optimization for Circular Dichroism Spectroscopy of Proteins: A Practical Approach for Rapid Acquisition of High-Quality Spectra
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DOI：10.1016/j.ab.2026.116134.png)
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Abstract
En 中文
• Systematic optimization of circular dichroism (CD) parameters (bandwidth, digital integration time, scan speed) reduced acquisition time by 10–20-fold while preserving spectral quality • Optimized settings provided reliable secondary structure estimation consistent with crystallographic data • The time required for thermal denaturation measurements at a single wavelength was reduced from 70 to 18 min, and that for temperature-ramping CD measurements from 270 to 90 min, enabling rapid stability evaluation • Multivariate analysis of temperature-ramping spectra allowed not only rapid evaluation of rituximab stability but also revealed that aggregation-induced structural changes occurred immediately after denaturation
Keywords:
Circular dichroism spectroscopy
Higher-order structure
Protein
Peptide
Biopharmaceutical
Antibody drug
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