.png){kind=icon}
.png){kind=icon}
Return
Pathways of thymidine hypermodification
.png){kind=icon}
OA
AI
DOI：10.1093/nar/gkab781.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
The DNAs of bacterial viruses are known to contain diverse, chemically complex modifications to thymidine that protect them from the endonuclease-based defenses of their cellular hosts, but whose biosynthetic origins are enigmatic. Up to half of thymidines in the Pseudomonas phage M6, the Salmonella phage ViI, and others, contain exotic chemical moieties synthesized through the post-replicative modification of 5-hydroxymethyluridine (5-hmdU). We have determined that these thymidine hypermodifications are derived from free amino acids enzymatically installed on 5-hmdU. These appended amino acids are further sculpted by various enzyme classes such as radical SAM isomerases, PLP-dependent decarboxylases, flavin-dependent lyases and acetyltransferases. The combinatorial permutations of thymidine hypermodification genes found in viral metagenomes from geographically widespread sources suggests an untapped reservoir of chemical diversity in DNA hypermodifications.
Keywords:
BACILLUS-SUBTILIS
RADICAL SAM
ALPHA-PUTRESCINYLTHYMINE
DEOXYRIBONUCLEIC-ACID
BACTERIOPHAGE DNA
BIOSYNTHESIS
TRIPHOSPHATE
RESTRICTION
MECHANISM
ENZYMES
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
13.1
Papers:
3.6W
Citations:
29.0W
Organization
View more organizations