.png){kind=icon}
Return
PDMS Microwell Stencil Based Multiplexed Single-Cell Secretion Analysis
.png){kind=icon}
PRE
AI
DOI：10.1002/pmic.201900231.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Multiplexed single-cell protein secretion analysis provides an in-depth understanding of cellular heterogeneity in intercellular communications mediated by secreted proteins in both fundamental and clinical research. However, it has been challenging to increase the proteomic parameters co-profiled from every single cell in a facile way. Herein, a simple method to improve the multiplexed proteomic parameters of PDMS microwell based single-cell secretion analysis platform by sandwiching PDMS stencil in between two antibody-coated glass slides is introduced. Two different antibody panels can be immobilized easily by static coating, without using sophisticated fluid handling or bulky equipment. 5-plexed, 3-fluorescence color single-cell secretion assay is demonstrated with this platform to investigate human monocytic U937 cells in response to lipopolysaccharide and phorbol myristate acetate stimulation, which identified the existence of functional subsets dictated by different cytokine profiles. The technology introduced here is simple, easy to operate, which holds great potential to become a powerful tool for profiling multiplexed single-cell cytokine secretion at high throughput to dissect cellular heterogeneity in secretome signatures.
Keywords:
multiplexed
PDMS microwell stencil
secretion analysis
single-cell analysis
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
3.9
Papers:
7.6K
Citations:
1.1W
Organization
Cited Papers
Pentobarbital and EEG Burst Suppression in Treatment of Status Epilepticus Refractory to Benzodiazepines and Phenytoin.png)
Epilepsia
EpilepsiaIF0
PREAI
ShareSaveShareSaveChemical Methods for the Simultaneous Quantitation of Metabolites and Proteins from Single Cells
OAAI
OAAIShareSaveShareSaveHigh-Throughput Secretomic Analysis of Single Cells to Assess Functional Cellular HeterogeneityANALYTICAL CHEMISTRYOAAI
ANALYTICAL CHEMISTRYIF6.7
OAAIShareSavePaper-Based 3D Scaffold for Multiplexed Single Cell Secretomic AnalysisANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
ShareSave
View more