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Peroxidase-like CuFeS2 nanoclusters coupled with glucose oxidase signal amplification for colorimetric immunodetection of carcinoembryonic antigen
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DOI：10.1007/s00604-026-08337-4.png)
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Abstract
En 中文
We constructed a sandwich-format colorimetric assay in which CuFeS2 nanoclusters act as an H2O2-responsive nanozyme and AuNPs bearing both glucose oxidase (GOx) and the detection antibody (Ab2) serve as the recognition-linked signal generator. Following capture of CEA on a capture antibody (Ab1)-coated well, the bound GOx-AuNPs-Ab2 probe oxidizes glucose and produces H2O2 locally. CuFeS2 then uses the peroxide to drive conversion of 3,3′,5,5′-tetramethylbenzidine (TMB) into its blue oxidized form, so the absorbance output scales with the amount of captured antigen. Under optimized conditions, the response was linear from 0.05 to 40 ng mL-1 and the detection limit was 22 pg mL-1. The assay gave acceptable repeatability, retained 91% of its initial response after one month at 4 °C, and discriminated CEA from the tested interferents. Preliminary measurements of a limited number of serum specimens were consistent with commercial ELISA results, and spike-and-recovery tests further supported the applicability of the assay in a serum matrix. These findings establish a CuFeS2/GOx cascade readout for visual and quantitative CEA determination, while further validation with larger clinical sample cohorts is still required.
Keywords:
CuFeS2 nanoclusters
Peroxidase-like activity
Glucose oxidase
Colorimetric immunoassay
Carcinoembryonic antigen
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