.png){kind=icon}
.png){kind=icon}
Return
Phase Separation of DNA-Encoded Artificial Cells Boosts Signal Amplification for Biosensing
.png){kind=icon}
PRE
AI
DOI：10.1002/anie.202306691.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Life-like hierarchical architecture shows great potential for advancing intelligent biosensing, but modular expansion of its sensitivity and functionality remains a challenge. Drawing inspiration from intracellular liquid-liquid phase separation, we discovered that a DNA-encoded artificial cell with a liquid core (LAC) can enhance peroxidase-like activity of Hemin and its DNA G-quadruplex aptamer complex (DGAH) without substrate-selectivity, unlike its gelled core (GAC) counterpart. The LAC is easily engineered as an ultrasensitive biosensing system, benefiting from DNA & PRIME;s high programmability and unique signal amplification capability mediated by liquid-liquid phase separation. As proof of concept, its versatility was successfully demonstrated by coupling with two molecular recognition elements to monitor tumor-related microRNA and profile cancer cell phenotypes. This scalable design philosophy offers new insights into the design of next generation of artificial cells-based biosensors.
Keywords:
Aptamers
Artificial Cell
Biosensing
Liquid-Liquid Separation
Signal Amplification
Journal
IF:
16.9
Papers:
5.6W
Citations:
53.0W