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Photon-Upconversion Barcoding with Multiple Barcode Channels: Application for Droplet Microfluidics
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DOI：10.1021/acs.analchem.9b03117.png)
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Abstract
En 中文
Barcoding facilitates high-throughput analytical methods in complex matrixes with a reduced volume of sample, reagents, time, and cost. Because of orthogonality to fluorescence, photon-upconversion barcodes attracted considerable attention in recent years. We constructed an epiluminescence detector, which, for the first time, demonstrated the reading of photon-upconversion spectra from microdroplets in a microfluidic chip with frequency up to 10 Hz. Non-negative least-squares deconvolution enabled the reading of an unprecedented number of photon-upconversion barcode channels (six) from emission spectra (excitation 980 nm, emission 430-875 nm). The standard deviation of barcode reading from microdroplets was similar to 1%. Described barcoding can be, for example, used for multiparameter titrations, multiplexed biological and chemical assays, optimizations on a microfluidic platform, and preparation of barcoded concentration gradients and libraries.
Keywords:
UPCONVERTING NANOPARTICLES
TRANSCRIPTOMICS
DISCOVERY
PARTICLES
PHASE
CELLS
ASSAY
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