.png){kind=icon}
.png){kind=icon}
Return
Plasmonic nanocrystals on polycarbonate substrates for direct and label-free biodetection of Interleukin-6 in bioengineered 3D skeletal muscles
.png){kind=icon}
OA
AI
G
J
M
J
E
J
DOI：10.1515/nanoph-2021-0426.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
The development of nanostructured plasmonic biosensors has been widely widespread in the last years, motivated by the potential benefits they can offer in inte-gration, miniaturization, multiplexing opportunities, and enhanced performance label-free biodetection in a wide field of applications. Between them, engineering tissues represent a novel, challenging, and prolific application field for nanostructured plasmonic biosensors considering the previously described benefits and the low levels of secreted biomarkers (approximate to pM-nM) to detect. Here, we present an integrated plasmonic nanocrystals-based biosensor us-ing high throughput nanostructured polycarbonate sub-strates. Metallic film thickness and incident angle of light for reflectance measurements were optimized to enhance the detection of antibody-antigen biorecognition events using numerical simulations. We achieved an enhancement in biodetection up to 3x as the incident angle of light de-creases, which can be related to shorter evanescent decay lengths. We achieved a high reproducibility between channels with a coefficient of variation below 2% in bulk refractive index measurements, demonstrating a high potential for multiplexed sensing. Finally, biosensing po-tential was demonstrated by the direct and label-free detection of interleukin-6 biomarker in undiluted cell cul-ture media supernatants from bioengineered 3D skeletal muscle tissues stimulated with different concentrations of endotoxins achieving a limit of detection (LOD) of approximate to 0.03 ng/mL (1.4 pM).
Keywords:
interleukin-6
label-free biosensing
plasmonic nanostructures
skeletal muscle
tissue engineering