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Polarized and structured light techniques for quantitative biomedical diagnostics
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DOI：10.1117/12.3090865.png)
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Abstract
En 中文
Here we showcase innovative optical methods for diagnosing amyloidosis and assessing macular health using polarized light microscopy (PLM) and structured light entoptics. We present a Congo red-free PLM approach to detect amyloid fibrils in hematoxylin and eosin (H&E)-stained biopsies from diverse tissues (abdominal subcutaneous, duodenal, thyroid, breast), quantifying birefringence and amyloid density via Fourier analysis with a 520 nm laser system. Results match Congo red-stained controls without artifacts, enabling rapid, objective diagnosis of amyloid-related diseases like Alzheimer's and cancer. Additionally, motivated by recent advances in structured light-based entoptic probes of macular health, we apply quantitative polarized light imaging to mouse retinal tissue to directly map birefringence and investigate the physical tissue interactions.
Keywords:
Structured light
polarized light microscopy
amyloidosis
macular degeneration
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