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Post-column derivatization high-performance liquid chromatography-ultraviolet-ultraviolet (HPLC-UV) method for the detection of Kryptofix 2.2.2 content in Positron Emission Tomography (PET) tracers
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DOI：10.1016/j.nucmedbio.2026.109610.png)
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Abstract
En 中文
A post-column derivatization high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed to determine Kryptofix 2.2.2 (K2.2.2) in PET tracers. This method utilizes the specific complexation between K2.2.2 and Pb2+, forming a stable 1:1 host-guest complex. The complexation produces a bathochromic shift in UV absorption maximum (from 210 nm to 254 nm), enabling sensitive detection. This approach addresses the limitations of poor performance and narrow applicability associated with direct single-method detection of K2.2.2. The chromatographic conditions were optimized (column flow rate: 0.7 mL & centerdot;min(-1); derivatization reagent flow rate: 0.7 mL & centerdot;min(-1); Pb2+ concentration: 50 mu g & centerdot;mL(-1)) and the method was validated. The results exhibited excellent linearity (1-100 mu g & centerdot;mL(-1), r(2) > 0.999), high reproducibility (relative standard deviation (RSD) < 5%), and a low detection limit (0.5 mu g & centerdot;mL(-1)). The method successfully quantified K2.2.2 in [F-18]FDG, [F-18]AV45 and [F-18]DPA714, achieving spiked recoveries of 89%-105%. Additionally, the method remained unaffected by interference from common sample components such as sodium chloride and sodium ascorbate. This method provides a novel and efficient quality control tool for the simultaneous analysis of radiochemical purity and K2.2.2 content in diverse PET tracers.
Keywords:
Kryptofix 2.2.2
Post-column derivatization HPLC
PET tracers
Quality control
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