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Preparation, characterization, and activity verification of Xylooligosaccharides from Lablab purpureus stalk
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DOI：10.2306/scienceasia1513-1874.2026.034.png)
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Abstract
En 中文
To promote the comprehensive utilization of underutilized Lablab purpureus stalk, this study innovatively employs it as a raw material to produce xylooligosaccharides (XOS) and evaluates their antioxidant and prebiotic activities. Hemicellulose B was extracted via alkali treatment and deproteinized using the Sevag method, as confirmed by UV-visible spectroscopy. High-performance liquid chromatography (HPLC) analysis after hydrolysis revealed the presence of 14 monosaccharides or derivatives, predominantly xylose and arabinose. XOS were prepared through xylanase hydrolysis and characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR). SEM revealed a cross-linked, opaque morphology; FT-IR confirmed the presence of beta-glycosidic bonds; and NMR indicated methyl/methoxy groups and 1 -> 4 glycosidic linkages. The XOS demonstrated stronger DPPH radical scavenging capacity and reducing power than hemicellulose B and effectively promoted the growth of Lactobacillus acidophilus and Bifidobacterium animalis. This work provides a novel approach for the high-value utilization of Lablab stalk.
Keywords:
Lablab
crop stalk
xylooligosaccharide
prebiotic activity
Journal
S
IF:
0.6
Papers:
75
Citations:
969
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