Prime-seq, efficient and powerful bulk RNA sequencing

doi:10.1186/s13059-022-02660-8

Prime-seq, efficient and powerful bulk RNA sequencing

2022-03-31

35

OA

AI

DOI：10.1186/s13059-022-02660-8

Original

Save

View PDF

Abstract

En 中文

Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost-efficient due to almost 50-fold cheaper library costs. We also validate a direct RNA isolation step, show that intronic reads are derived from RNA, and compare cost-efficiencies of available protocols. We conclude that prime-seq is currently one of the best options to set up an early barcoding bulk RNA-seq protocol from which many labs would profit.

Keywords:

RNA-seq

Transcriptomics

Genomics

Power analysis

Prime-seq, efficient and powerful bulk RNA sequencing

Abstract

AI Summary

Journal

Organization