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Principles for the design of multicellular engineered living systems
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DOI：10.1063/5.0076635.png)
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Abstract
En 中文
Remarkable progress in bioengineering over the past two decades has enabled the formulation of fundamental design principles for a variety of medical and non-medical applications. These advancements have laid the foundation for building multicellular engineered living systems (M-CELS) from biological parts, forming functional modules integrated into living machines. These cognizant design principles for living systems encompass novel genetic circuit manipulation, self-assembly, cell-cell/matrix communication, and artificial tissues/organs enabled through systems biology, bioinformatics, computational biology, genetic engineering, and microfluidics. Here, we introduce design principles and a blueprint for forward production of robust and standardized M-CELS, which may undergo variable reiterations through the classic design-build-test-debug cycle. This Review provides practical and theoretical frameworks to forward-design, control, and optimize novel M-CELS. Potential applications include biopharmaceuticals, bioreactor factories, biofuels, environmental bioremediation, cellular computing, biohybrid digital technology, and experimental investigations into mechanisms of multicellular organisms normally hidden inside the black box of living cells. (c) 2022 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
Keywords:
BLOOD-BRAIN-BARRIER
NUCLEAR HORMONE-RECEPTORS
CELL-FATE SPECIFICATION
PLURIPOTENT STEM-CELLS
GENE-EXPRESSION NOISE
TRANSCRIPTION FACTORS
ESCHERICHIA-COLI
EXTRACELLULAR-MATRIX
PRIMITIVE ENDODERM
ENDOTHELIAL-CELLS
Journal
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4.1
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491
Citations:
1.7K
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