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Production and accumulation of recombinant protein via vitellogenin transport pathway in Daphnia magna
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DOI：10.1016/j.jbiotec.2026.04.015.png)
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Abstract
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• A novel, low-cost expression system was established using Daphnia magna to produce and accumulate recombinant proteins in eggs. • Vitellogenin signal peptide (VtgSP) and beta-sheet domain (BS) were identified and used to mediate secretion into hemolymph and uptake into eggs. • The system enables simple purification by accumulating proteins in easily isolated eggs and represents the first in vivo analysis of vitellogenin-based transport in Daphnia.
Keywords:
recombinant protein
Daphnia magna
vitellogenin
expression system
egg accumulation
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