Return
Promoter methylation-associated brain-enriched long noncoding RNAs in glioblastoma: a multi-cohort public epigenomic re-analysis
.png){kind=icon}
OA
AI
Y
F
P
J
Y
A
P
DOI：10.1080/17501911.2026.2691031.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Glioblastoma (GBM) is epigenetically heterogeneous, and previous long noncoding RNA (lncRNA) methylation studies have often begun from tumor-wide screens. We asked whether brain-enriched lncRNAs show promoter methylation-associated suppression in GBM.
Brain-enriched lncRNAs were defined using GENCODE v49 and GTEx/UCSC Xena. Primary discovery used TCGA-GBM primary tumors with matched expression and promoter methylation; 52 patients had both data types. Spearman correlations identified methylation-associated candidates (ρ ≤−0.30; false discovery rate [FDR] < 0.05). External layers used GSE36278 methylation, CGGA expression/survival, and GSE131928 Smart-seq2 state scores.
Of 13,213 lncRNAs, 437 were brain-enriched and 76 entered matched TCGA analysis; seven met core criteria. PTPRD-AS1 and NFIB-AS1 showed the strongest inverse coupling (ρ = -0.665 and −0.639; FDR = 0.000016 and 0.000028). GSE36278 supported hypermethylation direction for all seven candidates, with six FDR-significant. MEG8 and FAM201A were downregulated in CGGA versus GTEx brain, and FAM201A associated with pseudobulk stemness and mesenchymal scores.
This public-data re-analysis prioritizes brain-enriched lncRNAs associated with promoter hypermethylation and lower expression in GBM, but does not establish direct methylation-mediated silencing.
Keywords:
Glioblastoma
brain-enriched long noncoding RNA
promoter methylation
methylation-expression association
public-data re-analysis
single-cell validation
Journal
IF:
2.6
Papers:
1.8K
Citations:
3.4K
Organization
Cited Papers