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Protein characterization of the IgM triplet involved in the diagnosis of congenital toxoplasmosis
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DOI：10.1051/parasite/2025065.png)
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Abstract
En 中文
Congenital toxoplasmosis is a fetal infection resulting from the transplacental transmission of Toxoplasma gondii in mothers who seroconvert during pregnancy. Neonatal diagnosis has recently been improved through the identification by L'Ollivier et al. (2012) and Peyclit et al. (2023) of a pathognomonic marker for congenital toxoplasmosis: the IgM triplet, corresponding to three high molecular weight bands of 75, 90, and 100 kDa, respectively found on the mother-child immunoblot pair profile. This is a new concept, as these three IgM bands reflect an immune response targeting proteins involved in vertical transmission of T. gondii. These proteins may be T. gondii secreted or non-secreted effectors implicated in host cell invasion, immune modulation, and parasite virulence. In this study, immunoproteomic techniques allowed us to identify thirty-two relevant protein spots on immunoblot, including four specifically associated with the IgM triplet. Protein identification by LC-MS/MS revealed several T. gondii proteins as strong candidates for the IgM triplet. Each of these proteins is, directly or indirectly, involved in cellular invasion and may also play a role in transplacental transmission of T. gondii. Identifying these proteins opens several avenues of therapeutic research that could improve the management of congenital toxoplasmosis. La toxoplasmose cong & eacute;nitale est une infection f oe tale r & eacute;sultant de la transmission transplacentaire de Toxoplasma gondii chez des m & egrave;res s & eacute;roconverties pendant la grossesse. Le diagnostic n & eacute;onatal a r & eacute;cemment & eacute;t & eacute; am & eacute;lior & eacute; gr & acirc;ce & agrave; l'identification par L'Ollivier et al. (2012) et Peyclit et al. (2023) d'un marqueur pathognomonique de la toxoplasmose cong & eacute;nitale : la triplette IgM, correspondant & agrave; trois bandes de haut poids mol & eacute;culaire de 75, 90 et 100 kDa respectivement, pr & eacute;sentes sur le profil de paires d'immunoblots m & egrave;re-enfant. Il s'agit d'un concept nouveau, car ces trois bandes IgM refl & egrave;tent une r & eacute;ponse immunitaire ciblant des prot & eacute;ines impliqu & eacute;es dans la transmission verticale de T. gondii. Ces prot & eacute;ines pourraient & ecirc;tre des effecteurs s & eacute;cr & eacute;t & eacute;s ou non par T. gondii impliqu & eacute;s dans l'invasion des cellules h & ocirc;tes, la modulation immunitaire et la virulence du parasite. Dans cette & eacute;tude, les techniques d'immunoprot & eacute;omique nous ont permis d'identifier trente-deux spots prot & eacute;iques pertinents sur immunoblot, dont quatre sp & eacute;cifiquement associ & eacute;s & agrave; la triplette IgM. Pour ces spots, l'identification des prot & eacute;ines par LC-MS/MS a r & eacute;v & eacute;l & eacute; que plusieurs prot & eacute;ines de T. gondii & eacute;taient de bonnes candidates pour la triplette IgM. Chacune de ces prot & eacute;ines est impliqu & eacute;e, directement ou indirectement, dans l'invasion cellulaire et pourrait & eacute;galement jouer un r & ocirc;le dans la transmission transplacentaire de T. gondii. L'identification de ces prot & eacute;ines ouvre de nouvelles perspectives th & eacute;rapeutiques susceptibles d'am & eacute;liorer la prise en charge de la toxoplasmose cong & eacute;nitale.
Keywords:
Toxoplasma gondii
Congenital toxoplasmosis
IgM triplet
Immunoproteomics
2-DE Immunobloting
LC-MS/MS
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