Pulsed laser irradiation induces the generation of alloy cluster ions for the screening of protease activity

doi:10.1016/j.bios.2022.114615

Pulsed laser irradiation induces the generation of alloy cluster ions for the screening of protease activity

2022-11-01

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DOI：10.1016/j.bios.2022.114615

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Pulsed laser irradiation can cause the fragmentation of nanoparticles, which generates cluster ions. This allows nanoparticles to be adopted as mass tag/signal amplifiers in laser desorption/ionization mass spectrometry (LDI-MS) bioassays. Herein, we demonstrate the potential of using the signal from alloy cluster ions in bioassays through a fibrin clot model to determine the activity of thrombin. A mixed solution of silver and gold nano -particles functionalized with fibrinogen (Fg-Ag NPs/Fg-Au NPs) treated with thrombin can form clots composed of aggregated fibrin-Au NPs/Ag NPs. These clots analyzed with LDI-MS are noted to form intense Ag-Au alloy cluster ions, especially [Ag2Au](+), which were used to detect thrombin concentration with a dynamic range of 2.5-50 pM in human plasma. This sensing platform was further employed for the screening of direct thrombin inhibitors. This work developed a novel bioassay utilizing metallic gas-phase reactions generated from pulsed laser irradiation of aggregated nanoparticles to monitor enzymatic activity and to screen inhibitors. We believe that LDS-MS can serve as a new platform for gas-phase reaction-based bioassays.

Keywords:

Metal nanoparticles

Laser irradiation

Gas -phase reaction

Cluster ions

Protease activity

Inhibitor screening

Pulsed laser irradiation induces the generation of alloy cluster ions for the screening of protease activity

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