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Quantifying Experimental Errors in Measuring Colloidal Interaction Potentials with Optical Tweezers
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DOI：10.1039/D5SM00551E.png)
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Abstract
En 中文
We present a systematic study on the measurement of pair interaction potentials between small particles using optical tweezers (OT); focusing on the modelling and quantification of three key experimental errors: z-motion error; dynamic error; and static error. While these errors have been previously acknowledged; their individual effects on such measurements had not been thoroughly examined. We develop a framework to model these errors and validate it through controlled experiments. By carefully tuning experimental parameters; we decouple and quantify each error source; demonstrating that they can be independently controlled and accounted for. Our approach enables a more precise access to the true interaction potential; reducing measurement ambiguities and improving the accuracy of comparisons with theoretical models. As a demonstration of the framework’s applicability; we apply our correction method to extract the depletion attraction potential from experimentally measured data; showcasing how systematic error removal enables the retrieval of physically meaningful interaction potentials. This work provides a robust methodology for enhancing the accuracy of OT-based potential measurements and for studying colloidal interactions.
Keywords:
optical tweezers
pair interaction potentials
systematic errors
colloidal interactions
depletion attraction
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