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Random access parallel microscopy

delete2021-01-12
delete10
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OA
AI
M
Mishal Ashraf
S
Sharika Mohanan
S
Sim, Byu Ri
T
Tam, Anthony
R
Rahemipour, Kiamehr
B
Brousseau, Denis
S
Simon Thibault
A
Alexander D. Corbett *
G
Gil Bub *
DOI:10.7554/eLife.56426delete
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Abstract

Abstract

En 中文
We introduce a random-access parallel (RAP) imaging modality that uses a novel design inspired by a Newtonian telescope to image multiple spatially separated samples without moving parts or robotics. This scheme enables near-simultaneous image capture of multiple petri dishes and random-access imaging with sub-millisecond switching times at the full resolution of the camera. This enables the RAP system to capture long-duration records from different samples in parallel, which is not possible using conventional automated microscopes. The system is demonstrated by continuously imaging multiple cardiac monolayer and Caenorhabditis elegans preparations.
Keywords:
WIDE-FIELD

Journal

eLife cover
eLife
IF:
0
Papers:
1.8W
Citations:
16

Organization

L
laval university
Scholars:
2.5W
Papers: 2.2W
Citations: 96
U
University of Exeter
Scholars:
2.0W
Papers: 2.1W
Citations: 3.6W