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Rapid detection of the oil palm bunch rot pathogen Paramarasmius palmivorus by recombinase polymerase amplification
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DOI：10.1016/j.pmpp.2026.103365.png)
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Abstract
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• RPA-flocculation enabled rapid detection of Paramarasmius palmivorus in oil palm. • Species-specific primers amplified target DNA within 15 min at 42 °C using RPA. • No cross-reactivity was observed with 36 non-target fungal species or host DNA. • The assay achieved a detection limit of 10 pg/μL genomic DNA. • Simple DNA extraction to RPA-flocculation workflow completed within 70 min.
Keywords:
Bridging flocculation
Fungal pathogen
Internal transcribed spacer
Isothermal amplification
Marasmiaceae
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